Dataset Information


Trivalent arsenic inhibits the functions of chaperonin complex

ABSTRACT: The goal of this study was to identify yeast mutants with arsenite hypersensitivity. Keywords: dose response Matched pools of yeast knockout (YKO) mutants were grown in paired growth media lacking or containing arsenite at various concentrations. The relative abundances of the YKO mutants were profiled using microarrays. Three types of pools were used in this study: (1) heterozygous diploid mutants; (2) haploid mutants obtained as progeny from the heterozygous diploid pool; and (3) haploid mutants also containing a null mutation of the prefoldin gene PFD1.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Daniel S Yuan   Xiaoling Wang  J M McCaffery  Zhiwei Huang  Judith Frydman  Jef D Boeke  Nick R Douglas  Xuewen Pan  Daniel Yuan  Stefanie Reissman 

PROVIDER: E-GEOD-5973 | ArrayExpress | 2010-07-26



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The exact molecular mechanisms by which the environmental pollutant arsenic works in biological systems are not completely understood. Using an unbiased chemogenomics approach in Saccharomyces cerevisiae, we found that mutants of the chaperonin complex TRiC and the functionally related prefoldin complex are all hypersensitive to arsenic compared to a wild-type strain. In contrast, mutants with impaired ribosome functions were highly arsenic resistant. These observations led us to hypothesize tha  ...[more]

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