Transcriptomics

Dataset Information

4

Perturbation of the Notch pathway in Xenopus laevis embryos


ABSTRACT: The Notch signaling pathway functions in a number of processes during embryologic development, especially the maintenance or aquisition of cell fate. We purturb the Notch signalling pathway in embryonic Xenopus laevis in order to 1) better characterize the downstream targets of Notch signalling, and 2) determine the extent to which early embryos can recover from transient purturbations to critical signalling pathways, if at all. Xenopus laevis embryos were unilaterally injected at the two cell stage with either GFP, GFP and ICD (Notch intracellular domain, an up-regulator of the Notch pathway), or GFP and DBM (domain-binding mutant, a downregulator of the Notch pathway). At stages 18, 28, and 38, for each injection, pooled total RNA from 10 embryos was extracted. Extraction was performed for three biological replicates for each time/injection condition. cDNA from total RNA was hybridized on Affymetrix Xenopus laevis Genome 2.0 arrays.

ORGANISM(S): Xenopus laevis  

SUBMITTER: Samuel Clamons   Brian Rabe  Margaret S Saha  Margaret Saha  Molly McDonough 

PROVIDER: E-GEOD-60127 | ArrayExpress | 2014-08-06

SECONDARY ACCESSION(S): GSE60127PRJNA257524

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2009-03-01 | E-MEXP-2058 | ArrayExpress
| PRJNA257524 | ENA
2009-09-15 | E-GEOD-9173 | ArrayExpress
2009-08-19 | GSE9173 | GEO
2014-08-04 | E-GEOD-56586 | ArrayExpress
| GSE92382 | GEO
2016-07-01 | E-GEOD-83340 | ArrayExpress
2009-09-15 | E-GEOD-9175 | ArrayExpress
2015-12-09 | E-GEOD-58420 | ArrayExpress
2009-03-01 | E-MEXP-2059 | ArrayExpress