Genomics,Multiomics

Dataset Information

96

An alternative pluripotent state confers interspecies chimaeric competency [ChIP-seq]


ABSTRACT: Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed ChIP-seqencing experiments using antibodies against H3K4me3 and H3K27me3, comparing conventional EpiSCs and rsEpiSCs, as well as comparing conventional human H1 ESCs and human H1 ESCs cultured in mouse rsEpiSCs based culture conditions (H1 rsESCs). Examination of 2 different histone modifications (H3K4me3, H3K27me3) in 2 pluripotent stem cell types in mouse and human.

ORGANISM(S): Musculus  

SUBMITTER: Jun Wu  Christopher Benner    

PROVIDER: E-GEOD-60604 | ArrayExpress| 2015-05-22

SECONDARY ACCESSION(S): GSE60604SRP045674PRJNA258642

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture paramet  ...[more]

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