Transcriptomics

Dataset Information

0

Targeting the MLL complex in castration-resistant prostate cancer [expression]


ABSTRACT: Resistance to androgen deprivation therapies and increased androgen receptor (AR) activity are major drivers of castration-resistant prostate cancer (CRPC). Prior work has focused on targeting AR directly; however, the identification and targeting of co-activators of AR signaling remains an underexplored area. Here we demonstrate that the MLL (mixed-lineage leukemia) complex, a well-known contributor in MLL-fusion-positive leukemia, acts as a co-activator of AR signaling. AR interacts with the MLL complex via its subunit, menin. Small molecule inhibition of the menin-MLL interaction blocks AR signaling and inhibits tumor growth in vivo. Furthermore, we find that menin is up-regulated in CRPC and high expression correlates with poor overall survival. Our study identifies the MLL complex as a co-activator of AR that can be targeted in advanced prostate cancer. ASH2L / Menin / MLL1 were knocked down using shRNA /siRNA in two prostate cancer cell lines, VCaP and LNCaP.

ORGANISM(S): Homo sapiens  

SUBMITTER: Yang Shi   Marcin Piotr Cieslik  Rohit Malik  Xiaojun Jing 

PROVIDER: E-GEOD-60836 | ArrayExpress | 2015-03-02

SECONDARY ACCESSION(S): GSE60836PRJNA259651

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2012-05-22 | E-GEOD-35988 | ArrayExpress
| GSE80450 | GEO
2016-05-22 | E-GEOD-80256 | ArrayExpress
2016-05-22 | E-GEOD-80450 | ArrayExpress
| GSE80256 | GEO
| GSE58478 | GEO
2014-09-10 | E-GEOD-58478 | ArrayExpress
2012-10-02 | E-GEOD-40050 | ArrayExpress
2016-06-30 | E-GEOD-61630 | ArrayExpress
2011-08-15 | E-GEOD-31410 | ArrayExpress