Transcriptomics

Dataset Information

30

Clostridium acetobutylicum ATCC 824 time course with Agilent Arrays


ABSTRACT: Using our newly designed C. acetobutylicum arrays we analyzed the mRNA changes during a time course experiment (up to 66 h) paying special attention on the study of the late timepoints. Keywords: time course Samples were taken from a Bioflow II reactor at 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 44, 48, 54, 58, and 66 hrs after bioreactor inoculation. Two slides per timepoint were hybridized on a dye swap configuration. The control mRNA was generated by combining samples taken at 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 44, 48, 54, 58, and 66 hrs after bioreactor inoculation. For timepoints 6, 8, 14, 32, 38, 66 our internal quality control check suggested a larger than normal variation of the intensities in the control channel and a third slide was hybridized. The discarded slides are GSM141189, GSM141203, GSM141222, GSM141229 and GSM141242.

ORGANISM(S): Clostridium acetobutylicum  

SUBMITTER: Ryan Sillers  Eleftherios T Papoutsakis   Nathan Cheng   Carlos J Paredes   Shawn W Jones    

PROVIDER: E-GEOD-6094 | ArrayExpress| 2014-05-02

SECONDARY ACCESSION(S): GSE6094PRJNA97653

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

The transcriptional program underlying the physiology of clostridial sporulation.

Jones Shawn W SW   Paredes Carlos J CJ   Tracy Bryan B   Cheng Nathan N   Sillers Ryan R   Senger Ryan S RS   Papoutsakis Eleftherios T ET  

Genome biology 20080716 7


Clostridia are ancient soil organisms of major importance to human and animal health and physiology, cellulose degradation, and the production of biofuels from renewable resources. Elucidation of their sporulation program is critical for understanding important clostridial programs pertaining to their physiology and their industrial or environmental applications.Using a sensitive DNA-microarray platform and 25 sampling timepoints, we reveal the genome-scale transcriptional basis of the Clostridi  ...[more]

Similar Datasets

2014-05-01 | E-GEOD-14433 | ArrayExpress
2014-05-01 | E-GEOD-14590 | ArrayExpress
2014-05-03 | E-GEOD-40954 | ArrayExpress
2014-05-02 | E-GEOD-6667 | ArrayExpress
2014-05-01 | E-GEOD-15384 | ArrayExpress
2014-05-02 | E-GEOD-7571 | ArrayExpress
2014-05-02 | E-GEOD-7572 | ArrayExpress
2014-05-03 | E-GEOD-4974 | ArrayExpress
2014-05-02 | E-GEOD-6607 | ArrayExpress
2014-05-03 | E-GEOD-4114 | ArrayExpress