Transcriptomics

Dataset Information

5

300 ppm Citrinin treatment for 2 h (GPL4399)


ABSTRACT: The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. Citrinin [518-75-2], 4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6-oxo-3H-2-benzopyran-7-crboxylic acid, is the one of the popular mycotoxicin produced by Penicillium and Aspergillus family possibly spread all over the world. This natural chemicals is one of the well characterized mycotoxin but the information especially mechanism of toxic action is limited. We used two types of microarrays, one have ORF (Open reading Frame) fragment on the surface of the glass as probes and the other have probes as oligonucleotide probes on the microarray with 3 dimensions. We compared data properties and studied the toxicity of citrinin to yeast cells. Keywords: stress response Biological samples were prepared from 3 independent experiments. Hybridization were done triplicate in each sample-control set. Sample labeling were swapped between 1st hybridization (control = Cy5, sample = Cy3) and 2nd and 3rd hybridization (control = Cy3, sample = Cy5).

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Emiko Kitagawa   Hitoshi iwahashi  YoungKyung Kim  Yoshiteru Suzuki 

PROVIDER: E-GEOD-6101 | ArrayExpress | 2010-06-10

SECONDARY ACCESSION(S): GSE6101PRJNA104451

REPOSITORIES: GEO, ArrayExpress

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Publications

Evaluation of toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray.

Iwahashi Hitoshi H   Kitagawa Emiko E   Suzuki Yoshiteru Y   Ueda Youji Y   Ishizawa Yo-hei YH   Nobumasa Hitoshi H   Kuboki Yoshihide Y   Hosoda Hiroshi H   Iwahashi Yumiko Y  

BMC genomics 20070405


BACKGROUND: Mycotoxins are fungal secondary metabolites commonly present in feed and food, and are widely regarded as hazardous contaminants. Citrinin, one of the very well known mycotoxins that was first isolated from Penicillium citrinum, is produced by more than 10 kinds of fungi, and is possibly spread all over the world. However, the information on the action mechanism of the toxin is limited. Thus, we investigated the citrinin-induced genomic response for evaluating its toxicity. RESULTS:  ...[more]

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