Transcriptomics

Dataset Information

2

Effect of long-term vitamin D supplementation on colonic gene expression in IL-10 Knockout mice at young adulthood


ABSTRACT: Low vitamin D status has been implicated in the progression of inflammatory bowel disease (IBD). This study used interleukin (IL)-10 knockout (KO) mice, that develop an intestinal inflammation when housed in a non-sterile environment, to determine if supplementation with vitamin D throughout life impacts colonic gene expression. Results provide important information on the intestinal response to vitamin D in inflamed mice. Female IL-10 knock out mice were randomized to 25 (Low, L) or 5000 (High, H) IU vitamin D/kg of diet throughout pregnancy and lactation. At weaning, offspring received the same or opposite diet as their mother until age 3 months. This resulted in four vitamin D interventions: HH, HL, LH, or LL where the first letter represents the diet consumed by dams during pregnancy and lactation and the second letter represents the diet consumed by offspring from weaning through to 3 months of age. Global gene expression was analyzed in the proximal colon of 3 months old mice (n=6 per group, for a total of 24 samples; samples came from different litters and moms IDs are given in the samples table below). Samples were stored at -80C.

ORGANISM(S): Mus musculus  

SUBMITTER: Jianmin Chen   Wendy E Ward  Elena M Comelli  Kristina A Fielding  Andrea J Glenn  Elena Comelli 

PROVIDER: E-GEOD-61111 | ArrayExpress | 2015-01-02

SECONDARY ACCESSION(S): GSE61111PRJNA260265

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| GSE61111 | GEO
| GSE87845 | GEO
2018-01-29 | PXD001540 | Pride
2017-08-30 | E-MTAB-5496 | ArrayExpress
2013-08-13 | E-GEOD-49797 | ArrayExpress
2018-07-09 | GSE104029 | GEO
| GSE87844 | GEO
| GSE86270 | GEO
2015-07-29 | E-GEOD-71424 | ArrayExpress
2013-06-01 | E-GEOD-43038 | ArrayExpress