Transcriptomics

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Effects of the insulin degrading enzyme silencing on the transcriptome of HepG2 cells


ABSTRACT: Insulin degrading enzyme (IDE) is a major enzyme responsible for insulin degradation in the liver. The modulation of insulin degrading enzyme activity is hypothesized to be a link between T2DM and liver cancer. Results provide insight into role of IDE in proliferation and other cell functions. HepG2 cells were transfected with 96nM siRNA for IDE or AllStars Negative Control siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen). 16 h after transfection, cells were treated with 10 nM insulin (Sigma Aldrich) or vehicle for 24 h in serum starvation condition. Total RNA was extracted. For each of the 4 conditions, 3 biological replicates were included.

ORGANISM(S): Homo sapiens  

SUBMITTER: Steven Dooley  Olga Pivovarova   Iryna Ilkavets   Carsten Sticht   Sergei Zhuk   Natalia Rudovich   Andreas F Pfeiffer    

PROVIDER: E-GEOD-61142 | ArrayExpress | 2014-09-06

SECONDARY ACCESSION(S): GSE61142PRJNA260342

REPOSITORIES: GEO, ArrayExpress

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