Transcriptomics

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Gene expression profile after Dax1 deletion in ES cells


ABSTRACT: To investigate the function of Dax1 in mouse ES cells, whole genome microarray expression profiling was employed to identify genes that are potentially affected by the deletion of Dax1. An inducible knockout ES cell line of Dax1 with the Cre-loxP system was generated and gene expression was measured (i) without Dax1 deletion, (ii) after 4 days of Dax1 deletion, or (iii) after several passags of Dax1 deletion. Each sample was prepared in triplicate.

ORGANISM(S): Mus musculus  

SUBMITTER: Hitoshi Niwa   Setsuko Fujii 

PROVIDER: E-GEOD-61152 | ArrayExpress | 2015-05-13

SECONDARY ACCESSION(S): GSE61152PRJNA260359

REPOSITORIES: GEO, ArrayExpress

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Publications

Nr0b1 is a negative regulator of Zscan4c in mouse embryonic stem cells.

Fujii Setsuko S   Nishikawa-Torikai Satomi S   Futatsugi Yoko Y   Toyooka Yayoi Y   Yamane Mariko M   Ohtsuka Satoshi S   Niwa Hitoshi H  

Scientific reports 20150316


Nuclear receptor subfamily 0, group B, member 1 (Nr0b1, also known as Dax1) is regarded as an important component of the transcription factor network that governs pluripotency in mouse embryonic stem (ES) cells. Here we generated inducible knockout ES cells for Nr0b1 using the Cre-loxP system and analyzed its precise function. We succeeded in establishing the Nr0b1-null ES cells and confirmed their pluripotency by showing their contribution to chimeric embryos. However, they proliferated slowly  ...[more]

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