Transcriptomics,Genomics

Dataset Information

307

Zic2, an enhancer-binding factor required for embryonic stem cell specification


ABSTRACT: The Zinc finger protein of the cerebellum 2 (Zic2) is one of the vertebrate homologs of the Drosophila pair-rule gene odd-paired (opa). Our molecular and biochemical studies have demonstrated that Zic2 to preferentially bind to transcriptional enhancers and functions as a cofactor that interacts with the NuRD complex in ES cells. Detailed genome-wide studies demonstrate that Zic2 function with Mbd3/NuRD in regulating the chromatin state and transcriptional output of genes linked to differentiation. Zic2 is dispensable for the selfrenewal of ES cells but is required for proper differentiation, similar to what has been previously reported for Mbd3/NuRD. Our study identifies Zic2 as a key factor in the execution of the pluripotency program with Mbd3/NuRD in ES cells. ChIP-seq of Zic2, Chd4, Mbd3 and Zic3 in mES cells. ChIP-seq of H3K27me3, H3K27ac, H3K4me3, H3K4me1 and PolII in mES cells after Zic2 shRNA and non-targeting shRNA. RNA-seq of mES cells after Zic2, Zic3, Mbd3 and Mta2 shRNA and non-targeting shRNA.

ORGANISM(S): Mus musculus  

SUBMITTER: Ali Shilatifard   Zhuojuan Luo  Xin Gao 

PROVIDER: E-GEOD-61188 | ArrayExpress | 2015-02-23

SECONDARY ACCESSION(S): SRP046299GSE61188PRJNA260473

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Zic2 is an enhancer-binding factor required for embryonic stem cell specification.

Luo Zhuojuan Z   Gao Xin X   Lin Chengqi C   Smith Edwin R ER   Marshall Stacy A SA   Swanson Selene K SK   Florens Laurence L   Washburn Michael P MP   Shilatifard Ali A  

Molecular cell 20150201 4


The Zinc-finger protein of the cerebellum 2 (Zic2) is one of the vertebrate homologs of the Drosophila pair-rule gene odd-paired (opa). Our molecular and biochemical studies demonstrate that Zic2 preferentially binds to transcriptional enhancers and is required for the regulation of gene expression in embryonic stem cells. Detailed genome-wide and molecular studies reveal that Zic2 can function with Mbd3/NuRD in regulating the chromatin state and transcriptional output of genes linked to differe  ...[more]

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