Genomics

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Change in H3K4me3 in Suz12(Bgal/Bgal) ESCs, and over Spinal Motor Neuron differentiation (ChIP-Seq)


ABSTRACT: Suz12(Bgal/Bgal) ESCs express a truncated form of Suz12 fused to Beta-galactosidase. These cells maintain a reduced level of H3K27me3 despite this mutation to a core component of PRC2, unlike Eed-/- ESCs whose H3K27me3 is ablated. This data shows the concomitant changes in H3K4me3 levels in these cells. An ESC line mutant in Suz12, a core component of Polycomb Repressive Complex 2, was assessed for H3K4me3 by ChIP-seq, as compared to a wild type ESC line, as well as both lines subjected to in vitro differentiation down the Spinal Motor Neuron pathway.

ORGANISM(S): Mus musculus  

SUBMITTER: Seraphim R Thornton   Laurie A Boyer 

PROVIDER: E-GEOD-61248 | ArrayExpress | 2014-11-17

SECONDARY ACCESSION(S): GSE61248SRP046724PRJNA260591

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Polycomb Repressive Complex 2 regulates lineage fidelity during embryonic stem cell differentiation.

Thornton Seraphim R SR   Butty Vincent L VL   Levine Stuart S SS   Boyer Laurie A LA  

PloS one 20141021 10


Polycomb Repressive Complex 2 (PRC2) catalyzes histone H3 lysine 27 tri-methylation (H3K27me3), an epigenetic modification associated with gene repression. H3K27me3 is enriched at the promoters of a large cohort of developmental genes in embryonic stem cells (ESCs). Loss of H3K27me3 leads to a failure of ESCs to properly differentiate, making it difficult to determine the precise roles of PRC2 during lineage commitment. Moreover, while studies suggest that PRC2 prevents DNA methylation, how thes  ...[more]

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