Transcriptomics

Dataset Information

298

Single-cell transcriptome analyses reveal signals to activate dormant neural stem cells.


ABSTRACT: Through single cell transcriptome analysis, we uncovered molecular signatures of CD133+/GFAP- ependymal (E) cells, CD133+/GFAP+ neural stem (B) cells, Dlx2+ neuroblasts (A cells), and Sox10+ oligodendrocyte progenitors (O cells) in the adult mouse forebrain neurogenic zone. prominent hub genes of the gene network unique to ependymal CD133+/GFAP- quiescent cells are enriched for receptors of angiogenic factors and immune-responsive genes. Administration of VEGF activated CD133+ ependymal stem cells lining not only the lateral, but also the 4th ventricles, and together with bFGF, elicited subsequent neural lineage differentiation and migration. Examination of 28 single cells and 4 populations of 10 cells from adult mouse forebrain neurogenic zone.

ORGANISM(S): Mus musculus  

SUBMITTER: Kunshan Zhang   Siguang Li  Yuping Luo  Yi E Sun 

PROVIDER: E-GEOD-61288 | ArrayExpress | 2015-05-21

SECONDARY ACCESSION(S): SRP046983GSE61288PRJNA260673

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications


The scarcity of tissue-specific stem cells and the complexity of their surrounding environment have made molecular characterization of these cells particularly challenging. Through single-cell transcriptome and weighted gene co-expression network analysis (WGCNA), we uncovered molecular properties of CD133(+)/GFAP(-) ependymal (E) cells in the adult mouse forebrain neurogenic zone. Surprisingly, prominent hub genes of the gene network unique to ependymal CD133(+)/GFAP(-) quiescent cells were enr  ...[more]

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