Genomics,Multiomics

Dataset Information

90

B-Catenin Mediates the Development of Behavioral Resilience [ChIP-Seq]


ABSTRACT: Here we show that β-catenin mediates pro-resilient and anxiolytic effects in mice in the nucleus accumbens (NAc), a key brain reward region, an effect that is mediated by β-catenin signaling in D2-type medium spiny neurons (MSNs) specifically. Conversely, blocking β-catenin function in NAc promotes susceptibility to chronic stress, and we show evidence of robust suppression of β-catenin transcriptional activity in the NAc both of depressed humans examined postmortem as well as of mice that display a susceptible phenotype after chronic stress, with a converse upregulation in mice that are stress resilient. Using ChIP-seq, we demonstrate a global, genome-wide enrichment of β-catenin in the NAc of resilient mice, and specifically identify Dicer1—important in small RNA (e.g., microRNA [miRNA]) biogenesis—as a critical β-catenin target gene involved in mediating a resilient phenotype. Small RNA-seq after excising β-catenin from the NAc in the context of chronic stress reveals dynamic β-catenin-dependent miRNA regulation associated with resilience. Control: 2 samples, Resilient: 2 samples, Susceptible: 2 samples; DNA input: 1 sample.

ORGANISM(S): Mus musculus  

SUBMITTER: Li Shen  Li SHEN   Ning-Yi SHAO   Caroline DIAS   Eric NESTLER    

PROVIDER: E-GEOD-61294 | ArrayExpress| 2014-11-28

SECONDARY ACCESSION(S): SRP046987GSE61294PRJNA260681

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications


β-catenin is a multi-functional protein that has an important role in the mature central nervous system; its dysfunction has been implicated in several neuropsychiatric disorders, including depression. Here we show that in mice β-catenin mediates pro-resilient and anxiolytic effects in the nucleus accumbens, a key brain reward region, an effect mediated by D2-type medium spiny neurons. Using genome-wide β-catenin enrichment mapping, we identify Dicer1-important in small RNA (for example, microRN  ...[more]

Similar Datasets

2014-11-28 | E-GEOD-61295 | ArrayExpress
2016-08-07 | E-GEOD-85005 | ArrayExpress
2016-08-05 | E-GEOD-85136 | ArrayExpress
2015-09-26 | E-GEOD-54263 | ArrayExpress
2014-05-01 | E-GEOD-11939 | ArrayExpress
| GSE76977 | GEO
| GSE110725 | GEO
| GSE72343 | GEO
2018-07-31 | GSE107941 | GEO
2012-11-06 | E-GEOD-36005 | ArrayExpress