Transcriptomics

Dataset Information

36

Changes in cholesterol homeostasis and acute phase response following pulmonary exposure to multi-walled carbon nanotubes links carbon nanotube exposure to risk of cardiovascular disease


ABSTRACT: Adverse lung effects in rodents following pulmonary exposure to multi-walled carbon nanotubes (MWCNT) are well documented. However, systemic effects are less understood. Prospective epidemiological studies have shown increased cardiovascular disease risk after pulmonary exposure to airborne particles, which has led to concerns that inhalation exposure to MWCNT might pose similar risks. We used high-content genomics tools to compare hepatic responses after exposure to a short, entangled MWCNT to the hepatic responses after exposure to a long, stiffer MWCNT at the global transcriptomic level. Female C57BL/6 mice were exposed by single intratracheal instillation to 162 µg/mouse of a short MWCNT (NRCWE-26 (NC-7000), 847±102 nm in length) or long MWCNT (NM-401 (CP-0006-SG), 4048±366 nm in length). Liver tissues were harvested 24 h, 3 d and 28 d after exposure. This experiment examined the pulmonary transcriptional response of female C57BL/6 mice exposed to NRCWE-26, a short multi-walled carbon nanotube, and NM-401, a long multi-walled carbon nanotube, at three doses: D1 (18 μg), D2 (54 μg), D3 (162 μg), and vehicle control. Each dose group was examined 1, 3 or 28 days post-exposure. Each dose group had 6 biological replicates. There were a total of 72 samples included in the final analysis using a two-color reference design.

ORGANISM(S): Mus musculus  

SUBMITTER: Anne T Saber  Dongmei Wu   Sabina Halappanavar   Ulla Vogel   Håkan Wallin   Ole Andersen   Andrew Williams   Józef Szarek   Nicklas R Jacobsen   Sarah Søs Poulsen   Carole L Yauk   Alicja Mortensen    

PROVIDER: E-GEOD-61366 | ArrayExpress| 2015-02-07

SECONDARY ACCESSION(S): GSE61366PRJNA260884

REPOSITORIES: GEO, ArrayExpress

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Publications

Changes in cholesterol homeostasis and acute phase response link pulmonary exposure to multi-walled carbon nanotubes to risk of cardiovascular disease.

Poulsen Sarah S SS   Saber Anne T AT   Mortensen Alicja A   Szarek Józef J   Wu Dongmei D   Williams Andrew A   Andersen Ole O   Jacobsen Nicklas R NR   Yauk Carole L CL   Wallin Håkan H   Halappanavar Sabina S   Vogel Ulla U  

Toxicology and applied pharmacology 20150122 3


Adverse lung effects following pulmonary exposure to multi-walled carbon nanotubes (MWCNTs) are well documented in rodents. However, systemic effects are less understood. Epidemiological studies have shown increased cardiovascular disease risk after pulmonary exposure to airborne particles, which has led to concerns that inhalation exposure to MWCNTs might pose similar risks. We analyzed parameters related to cardiovascular disease, including plasma acute phase response (APR) proteins and plasma  ...[more]

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