Transcriptomics

Dataset Information

30

Transcription profiling of CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cells


ABSTRACT: Gene expression profiles of CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cells were compared. Comparison of expression profiles of hematopoietic stem cells from fetal liver, umbilical cord blood, bone marrow and mobilized pheripheral blood allowed us to identify a unique set of genes with conserved expression during ontogeny. Experiment Overall Design: CD34+CD38- en CD34+CD38+ cell populations were isolated by cell sorting from human Bone Marrow, mobilized peripheral blood, umbilical cord blood and fetal liver. Total RNA was isolated from each cell population followed by the synthesis of biotinylated cRNA. After fragmentation the biotinylated cRNA was hybridized to affymetrix U133A chips.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Homo sapiens  

SUBMITTER: Arnolda de Nooij-van Dalen  

PROVIDER: E-GEOD-6146 | ArrayExpress| 2011-06-10

SECONDARY ACCESSION(S): GSE6146PRJNA97683

REPOSITORIES: GEO, ArrayExpress

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