Transcriptomics,Multiomics

Dataset Information

4

Cooperativity, specificity and evolutionary stability of Polycomb targeting in Drosophila


ABSTRACT: We report the evolutionary behaviour of Polycomb group proteins, their recruitment factors and their underlying sequences by performing ChIP-seq analysis in 4-5 different Drosophila species (GSE60428) and HiC analysis in Drosophila melanogaster. We demonstrate an extremely high conservation of Polycomb repressive domains across Drosophila species We validate few cases of PRE divergence that shows that cis-driven PRE evolution is a rare event. We further show that PHO recruitment to Polycomb domains is evolutionarily robust to motif changes and that PRC1 stabilizes binding of its key recruiter HiC experiments in wild type drosophila embryos

OTHER RELATED OMICS DATASETS IN: PRJNA261249PRJNA258256

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Noa O Elkayam   Jia-Ming Chang  Marianne Entrevan  Bernd Schuettengruber  Giacomo Cavalli  Hugues Parrinello  Amos Tanay  Shani Stern  Aubin Thomas  Tom Sexton  Eitan Yaffe 

PROVIDER: E-GEOD-61471 | ArrayExpress | 2014-10-20

SECONDARY ACCESSION(S): SRP047189GSE61471PRJNA261249

REPOSITORIES: GEO, ArrayExpress, ENA

Dataset's files

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E-GEOD-61471.additional.1.zip Other
E-GEOD-61471.additional.2.zip Other
E-GEOD-61471.idf.txt Idf
E-GEOD-61471.sdrf.txt Txt
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Publications

Cooperativity, specificity, and evolutionary stability of Polycomb targeting in Drosophila.

Schuettengruber Bernd B   Oded Elkayam Noa N   Sexton Tom T   Entrevan Marianne M   Stern Shani S   Thomas Aubin A   Yaffe Eitan E   Parrinello Hugues H   Tanay Amos A   Cavalli Giacomo G  

Cell reports 20141002 1


Metazoan genomes are partitioned into modular chromosomal domains containing active or repressive chromatin. In flies, Polycomb group (PcG) response elements (PREs) recruit PHO and other DNA-binding factors and act as nucleation sites for the formation of Polycomb repressive domains. The sequence specificity of PREs is not well understood. Here, we use comparative epigenomics and transgenic assays to show that Drosophila domain organization and PRE specification are evolutionarily conserved desp  ...[more]

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