Transcriptomics

Dataset Information

297

Transcription profiling by high throughput sequencing of Arabidopsis roots, shoots, inflorescences, and siliques of wild type and clf-28 mutant plants


ABSTRACT: CURLY LEAF (CLF), the major histone methyltransferase of Polycomb Repressive Complex 2 (PRC2), modifies trimethylation of histone H3 lysine 27 (H3K27me3) and mediates dynamical chromatin repression in Arabidopsis. Here we used strand specific RNA-sequencing to profile Arabidopsis transcriptomes obtained from roots, shoots, flowers and siliques of Col-0 and clf-28 plants. Our analysis identified a large number of CLF-regulatedd transcripts in Arabidopsis. Transcriptome profiling in roots, shoots, inflorescences and siliques of WT and clf-28 plants with 3 biological replicates.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Nam-Hai Chua   Shulin Deng  Jian Ye  Jun Liu  Huan Wang 

PROVIDER: E-GEOD-61545 | ArrayExpress | 2016-03-28

SECONDARY ACCESSION(S): SRP047300GSE61545PRJNA261433

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis.

Liu Jun J   Deng Shulin S   Wang Huan H   Ye Jian J   Wu Hui-Wen HW   Sun Hai-Xi HX   Chua Nam-Hai NH  

Plant physiology 20160304 1


CURLY LEAF (CLF), a histone methyltransferase of Polycomb Repressive Complex 2 (PRC2) for trimethylation of histone H3 Lys 27 (H3K27me3), has been thought as a negative regulator controlling mainly postgermination growth in Arabidopsis (Arabidopsis thaliana). Approximately 14% to 29% of genic regions are decorated by H3K27me3 in the Arabidopsis genome; however, transcriptional repression activities of PRC2 on a majority of these regions remain unclear. Here, by analysis of transcriptome profiles  ...[more]

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