Transcriptomics

Dataset Information

303

The neural crest is a source of mesenchymal stem cells with specialized hematopoietic stem-cell-niche function


ABSTRACT: Mesenchymal stem cells (MSCs) And osteolineage cells contribute to the hematopoietic stem cell (HSC) Niche in the bone marrow of long bones. However, Their developmental relationships remain unclear. Here we demonstrate that different MSC populations in the developing marrow of long bones have distinct functions. Proliferative mesoderm-derived nestin- MSCs participate in fetal skeletogenesis, And lose MSC activity soon after birth. In contrast, Quiescent neural-crest-derived nestin+ Cells in the same bones preserve MSC activity, But do not generate fetal chondrocytes. Instead, They differentiate into HSC-niche-forming MSCs, Helping to establish the HSC niche by secreting Cxcl12. Perineural migration of these cells to the bone marrow requires the ErbB3 receptor. The neonatal Nestin-GFP+ PDGFR- Cell population also contains Schwann-cell precursors, But does not comprise mature Schwann cells. Thus, In the developing bone marrow HSC-niche-forming MSCs share a common origin with sympathetic peripheral neurons and glial cells, And ontogenically distinct MSCs have non-overlapping functions in endochondrogenesis and HSC niche formation. Total RNA was isolated from small numbers of FACS sorted stromal cells, obtained from neonatal Nes-Gfp bone marrow preparations (2 biological replicates). Each independent set of samples was obtained from pooled skeletal elements (long bones and sterna) form multiple littermates.

ORGANISM(S): Mus musculus  

SUBMITTER: Carlos Torroja   Joan Isern  Fatima Sanchez-Cabo  Simón Méndez-Ferrer 

PROVIDER: E-GEOD-61695 | ArrayExpress | 2014-10-15

SECONDARY ACCESSION(S): GSE61695SRP047440PRJNA261856

REPOSITORIES: GEO, ArrayExpress, ENA

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The neural crest is a source of mesenchymal stem cells with specialized hematopoietic stem cell niche function.

Isern Joan J   García-García Andrés A   Martín Ana M AM   Arranz Lorena L   Martín-Pérez Daniel D   Torroja Carlos C   Sánchez-Cabo Fátima F   Méndez-Ferrer Simón S  

eLife 20140925


Mesenchymal stem cells (MSCs) and osteolineage cells contribute to the hematopoietic stem cell (HSC) niche in the bone marrow of long bones. However, their developmental relationships remain unclear. In this study, we demonstrate that different MSC populations in the developing marrow of long bones have distinct functions. Proliferative mesoderm-derived nestin(-) MSCs participate in fetal skeletogenesis and lose MSC activity soon after birth. In contrast, quiescent neural crest-derived nestin(+)  ...[more]

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