Transcriptomics

Dataset Information

296

RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation


ABSTRACT: Polyadenylated RNA from individual germinal vesicle and metaphase II ooyctes was amplified and processed for Illumina sequencing. Increases in polyadenylated transcript abundance were observed and are likely due to cytoplasmic polyadenylation. Transcriptomic analysis of GV and MII bovine oocytes.

ORGANISM(S): Bos taurus  

SUBMITTER: James L Chitwood   James Lee Chitwood  Pablo J Ross 

PROVIDER: E-GEOD-61717 | ArrayExpress | 2015-07-08

SECONDARY ACCESSION(S): SRP047451GSE61717PRJNA261946

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation.

Reyes Juan M JM   Chitwood James L JL   Ross Pablo J PJ  

Molecular reproduction and development 20150105 2


Molecular changes occurring during mammalian oocyte maturation are partly regulated by cytoplasmic polyadenylation (CP) and affect oocyte quality, yet the extent of CP activity during oocyte maturation remains unknown. Single bovine oocyte RNA sequencing (RNA-Seq) was performed to examine changes in transcript abundance during in vitro oocyte maturation in cattle. Polyadenylated RNA from individual germinal-vesicle and metaphase-II oocytes was amplified and processed for Illumina sequencing, pro  ...[more]

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