Genomics

Dataset Information

296

H3K27me3 ChIP-seq profiling in MCF7 cell lines under hypoxia


ABSTRACT: Purpose: Study hypoxia induced changes in genome-wide H3K27me3 occupancy Methods: Using the MCF7 breast epithelial adenocarcinoma cell line as a model, we studied epigenomic reprogramming as a function of fluctuating oxygen tension. To this end, we combined chromatin-immunoprecipitation and deep-sequencing analysis to identify H3K27me3-marks in MCF7 cells subjected to changes in oxygenation (i.e. acute hypoxia, chronic hypoxia). Results: H3K27me3-marks showed a rapid global increase at specific sites throughout the genome under hypoxia, both genic and inter-genic. Conclusions: Our data show that oxygen availability dynamically regulates the epigenetic state of the genome. Genome-wide H3K27me3-mark profiles were generated by combining ChIP analysis with deep sequencing using Illumina GAIIx.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michiel Emanuel Adriaens   Brad Wouters  Twan Van Den Beucken  Willem Voncken  Michiel Adriaens  Peggy Prickaerts 

PROVIDER: E-GEOD-61740 | ArrayExpress | 2014-09-26

SECONDARY ACCESSION(S): SRP047475GSE61740PRJNA262005

REPOSITORIES: GEO, ArrayExpress, ENA

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