Genomics

Dataset Information

297

Repression of the soma-specific transcriptome by Polycomb-repressive complex 2 promotes male germ cell development


ABSTRACT: Polycomb-repressive complex 2 (PRC2) catalyzes the methylation of histone H3 Lys27 (H3K27) and functions as a critical epigenetic regulator of both stem cell pluripotency and somatic differentiation, but its role in male germ cell development is unknown. Using conditional mutagenesis to remove the core PRC2 subunits EED and SUZ12 during male germ cell development, we identified a requirement for PRC2 in both mitotic and meiotic germ cells. We observed a paucity of mutant spermatogonial stem cells (SSCs), which appears independent of repression of the known cell cycle inhibitors Ink4a/Ink4b/Arf. Moreover, mutant spermatocytes exhibited ectopic expression of somatic lamins and an abnormal distribution of SUN1 proteins on the nuclear envelope. These defects were coincident with abnormal chromosome dynamics, affecting homologous chromosome pairing and synapsis. We observed acquisition of H3K27me3 on stage-specific genes during meiotic progression, indicating a requirement for PRC2 in regulating the meiotic transcriptional program. Together, these data demonstrate that transcriptional repression of soma-specific genes by PRC2 facilitates homeostasis and differentiation during mammalian spermatogenesis. Examination of two different types of histone modifications (H3K27me3 and H3K4me3) in spermatocytes at two different stages of development (P12 and P17) and the DNA binding protein, EED, in spermatocytes at one stage of development, P17. At P12, there was a single replicate of H3K27me3, H3K4me3 and input. At P17, there were two replicates of H3K27me3, H3K4me3 and input and a single replicate for EED.

ORGANISM(S): Mus musculus  

SUBMITTER: Joshua D Starmer   Joshua Starmer  Andrew Fedoriw  Terry Magnuson  Della Yee  Weipeng Mu 

PROVIDER: E-GEOD-61902 | ArrayExpress | 2014-10-01

SECONDARY ACCESSION(S): GSE61902SRP048529PRJNA262652

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Repression of the soma-specific transcriptome by Polycomb-repressive complex 2 promotes male germ cell development.

Mu Weipeng W   Starmer Joshua J   Fedoriw Andrew M AM   Yee Della D   Magnuson Terry T  

Genes & development 20140901 18


Polycomb-repressive complex 2 (PRC2) catalyzes the methylation of histone H3 Lys27 (H3K27) and functions as a critical epigenetic regulator of both stem cell pluripotency and somatic differentiation, but its role in male germ cell development is unknown. Using conditional mutagenesis to remove the core PRC2 subunits EED and SUZ12 during male germ cell development, we identified a requirement for PRC2 in both mitotic and meiotic germ cells. We observed a paucity of mutant spermatogonial stem cell  ...[more]

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