Transcriptomics

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Transcription profiling of E.coli biofilms treated with 7-hydroxyindole and isatin


ABSTRACT: For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:100. For EHEC with 7-hydroxyindole and isatin, 1000 mM 7-hydroxyindole in 250 mL DMF, 250 mM isatin in 250 mL DMF, or 250 mL DMF alone were added to cells grown in LB. The cells were shaken at 250 rpm and 30°C for 7 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm. Experiment Overall Design: Strain: EHEC EDL933 (ATCC 43895) wild type Experiment Overall Design: Medium: LB Experiment Overall Design: Cells: Suspension cells and biofilm cells on glass wool Experiment Overall Design: Time: 7h

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Escherichia coli  

SUBMITTER: Jintae Lee   

PROVIDER: E-GEOD-6195 | ArrayExpress | 2008-06-14

SECONDARY ACCESSION(S): GSE6195PRJNA100679

REPOSITORIES: GEO, ArrayExpress

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Publications

Enterohemorrhagic Escherichia coli biofilms are inhibited by 7-hydroxyindole and stimulated by isatin.

Lee Jintae J   Bansal Tarun T   Jayaraman Arul A   Bentley William E WE   Wood Thomas K TK  

Applied and environmental microbiology 20070504 13


Since indole is present at up to 500 microM in the stationary phase and is an interspecies biofilm signal (J. Lee, A. Jayaraman, and T. K. Wood, BMC Microbiol. 7:42, 2007), we investigated hydroxyindoles as biofilm signals and found them also to be nontoxic interspecies biofilm signals for enterohemorrhagic Escherichia coli O157:H7 (EHEC), E. coli K-12, and Pseudomonas aeruginosa. The genetic basis of EHEC biofilm formation was also explored, and notably, virulence genes in biofilm cells were re  ...[more]

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