Transcriptomics

Dataset Information

3

Follicular helper T cell signature in Type 1 Diabetes


ABSTRACT: This study set out to examine CD4 T cell differentiation in a mouse model of diabetes based on transgenic expression of ovalbumin under the control of the rat insulin promoter and co-expression of the DO11.10 transgene (DO11 x rip-mOVA mice). The transcriptome of T cells isolated from the pancreatic lymph nodes (lymph nodes draining the site of self antigen expression) was compared with that of T cells isolated from inguinal lymph nodes (non-draining lymph nodes). T cells were sorted based on expression of CD4, DO11.10 TCR (KJ-126), CD25 and CD69. Primary cells from 6 week old DO11 x rip-mOVA mice were isolated ex-vivo from the pancreatic lymph nodes or inguinal lymph nodes. Cells were sorted by flow cytometry using antibodies to CD4, DO11.10 TCR (KJ-126+), CD25 and CD69. 3-6 replicates were collected per experimental group with each replicate deriving from 14 mice. RNA was isolated using the RNeasy micro kit (Qiagen).

ORGANISM(S): Mus musculus  

SUBMITTER: Rupert Kenefeck   Lucy Walker  Masahiro Ono 

PROVIDER: E-GEOD-62104 | ArrayExpress | 2014-10-07

SECONDARY ACCESSION(S): GSE62104PRJNA263199

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2014-03-02 | E-GEOD-49507 | ArrayExpress
2013-05-15 | E-GEOD-46453 | ArrayExpress
2009-11-01 | E-MEXP-2237 | ArrayExpress
2010-12-31 | E-MEXP-2703 | ArrayExpress
| GSE71162 | GEO
| GSE109160 | GEO
2018-07-10 | GSE89481 | GEO
| GSE113249 | GEO
| GSE113250 | GEO
| GSE86971 | GEO