Transcriptomics,Multiomics

Dataset Information

3

Transcription profiling of uteri from gonadectomized C57BL/6 mice injected with 17-Estradiol (E2) to identify genes modulated by a physiological dose of E2 in uterus.


ABSTRACT: 17ß-Estradiol (E2) is well known to be associated with uterine cancer, endometriosis, and leiomyomas. Although insulin-like growth factor I (IGF-I) has been identified as a mediator of the uterotrophic effect of E2 in several studies, this mechanism is still not well understood. In the present study, identification of the genes modulated by a physiological dose of E2, in the uterus, has been done in ovariectomized mice using Affymetrix microarrays. The E2-induced genomic profile shows that multiple genes belonging to the IGF-I pathway are affected after exposure to E2. Two phases of regulation could be identified. First, from 0 to 6 h, the expression of genes involved in the cell cycle, growth factors, protein tyrosine phosphatases, and MAPK phosphatases is quickly upregulated by E2, while IGF-I receptor and several genes of the MAPK and phosphatidylinositol 3-kinase pathways are downregulated. Later, i.e., from 6 to 24 h, transporters and peptidases/proteases are stimulated, whereas defense-related genes are differentially regulated by E2. Finally, cytoarchitectural genes are modulated later. The present data show that a physiological dose of E2 induces, within 24 h, a series of transcriptional events that promote the uterotrophic effect. Among these, the E2-mediated activation of the IGF-I pathway seems to play a pivotal role in the uterotrophic effect. Furthermore, the protein tyrosine phosphatases and MAPK phosphatases are likely to modulate the estrogenic uterotrophic action by targeting, at different steps, the IGF-I pathway. Experiment Overall Design: Ten 10-11 weeks old C57Bl/6 mice were gonadectomized under isoflurane-induced anesthesia and sacrified seven days after GDX. Mice were injected s.c with E2 (0.05ug/mice) or vehicle, at 0.5, 1, 3, 6, 12 or 24 h before sacrifice. Uteri was removed and pooled before total RNA extraction. Samples was hybridized to U74Av2 Genechips (Affymetrix).

OTHER RELATED OMICS DATASETS IN: PRJNA97535

INSTRUMENT(S): 418 [Affymetrix]

SUBMITTER: Ezequiel L. Calvo  

PROVIDER: E-GEOD-6219 | ArrayExpress | 2007-11-14

SECONDARY ACCESSION(S): GDS2638GSE6219PRJNA97535

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-6219.README.txt Txt
E-GEOD-6219.eSet.r Other
E-GEOD-6219.idf.txt Idf
E-GEOD-6219.processed.1.zip Processed
E-GEOD-6219.raw.1.zip Raw
Items per page:
1 - 5 of 6
altmetric image

Publications

Temporal analysis of E2 transcriptional induction of PTP and MKP and downregulation of IGF-I pathway key components in the mouse uterus.

Ivanga Mahinè M   Labrie Yvan Y   Calvo Ezequiel E   Belleau Pascal P   Martel Céline C   Luu-The Van V   Morissette Jean J   Labrie Fernand F   Durocher Francine F  

Physiological genomics 20070301 1


17beta-Estradiol (E2) is well known to be associated with uterine cancer, endometriosis, and leiomyomas. Although insulin-like growth factor I (IGF-I) has been identified as a mediator of the uterotrophic effect of E2 in several studies, this mechanism is still not well understood. In the present study, identification of the genes modulated by a physiological dose of E2, in the uterus, has been done in ovariectomized mice using Affymetrix microarrays. The E2-induced genomic profile shows that mu  ...[more]

Similar Datasets

2007-01-01 | GSE6219 | GEO
2007-01-01 | S-EPMC2727866 | BioStudies
2012-01-01 | S-EPMC3474284 | BioStudies
1992-01-01 | S-EPMC1132746 | BioStudies
2008-01-01 | S-EPMC2631373 | BioStudies
2006-01-01 | S-EPMC1761141 | BioStudies
2011-01-01 | S-EPMC3156151 | BioStudies
2012-01-01 | S-EPMC3936881 | BioStudies
2018-01-01 | S-EPMC6538939 | BioStudies
2020-05-28 | GSE151242 | GEO