Transcriptomics

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Expression data from IL-1β-stimulated immature human enterocytes treated with probiotic-conditioned media


ABSTRACT: The conditioned media from Bifidobacterium infantis (BCM) and Lactobacillus acidophilus (LCM) were reported to promote maturation of innate immune response gene expression, which explained the protective effects of probiotics in clinical necrotizing enterocolitis. We used microarray analysis to investigate the expression of genes involved in regulation of BCM and LCM in IL-1β stimulated immature human enterocytes. The H4 cells (a human nontransformed primary intestinal epithelial cell line) were pretreated with BCM or LCM (15%) for 30 minutes, and then stimulated with or without IL-1β (10 ng/mL) for 4 h. Cell media and IL-1β stimulation were negative and positive control, respectively. RNA was extracted for mcroarray analysis. A total of six treatments (triplicates for each) were included in this study. As one RNA sample in IL-1β-stimulated cells treated with BCM was dropped due to bad quality, seventeen samples were analyzed. Various treatments were compared to the nagative control (cell media alone). Genes with a fold-change ≥2 and a p value ≤ 0.05 were selected.

ORGANISM(S): Homo sapiens  

SUBMITTER: William A Walker   Lei Lu  Ayla Ergun  Kriston Ganguli  Shuangshuang Guo  Yuming Guo 

PROVIDER: E-GEOD-62208 | ArrayExpress | 2015-06-22

SECONDARY ACCESSION(S): GSE62208PRJNA263475

REPOSITORIES: GEO, ArrayExpress

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Publications

Secreted Metabolites of Bifidobacterium infantis and Lactobacillus acidophilus Protect Immature Human Enterocytes from IL-1β-Induced Inflammation: A Transcription Profiling Analysis.

Guo Shuangshuang S   Guo Yuming Y   Ergun Ayla A   Lu Lei L   Walker W Allan WA   Ganguli Kriston K  

PloS one 20150423 4


Combination regimens of Bifidobacterium infantis and Lactobacillus acidophilus have been demonstrated to prevent necrotizing enterocolitis (NEC) in clinical trials. However, the molecular mechanisms responsible for this protective effect are not well understood. Additionally, conditioned media from individual cultures of these two probiotics show strain specific modulation of inflammation using in vitro human intestinal NEC models. Here we report a transcription profiling analysis of gene expres  ...[more]

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