Transcriptomics

Dataset Information

237

Small RNA Solexa sequencing of chickpea (Cicer arietinum)


ABSTRACT: The total RNA were extracted from pooled tissues of leaves and flowers from several plants of chickpea (Cicer arietinum) using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Then small RNAs ranging in 18–30 nucleotides were size fractionated electrophoretically, isolated from the gel, ligated with the 5′ and 3′ RNA adapters. The ligated product was reverse transcribed and subsequently amplified using 10–12 PCR cycles. The purified PCR product was sequenced using Illumina Genome Analyzer II. The qualified reads were used to predict microRNAs and phased small interfering RNAs from chickpea. Identification of microRNAs and phased small inferfering RNAs in chickpea (Cicer arietinum) by analyzing small RNA sequencing profiles of leaves and flowers using Illumina GAII.

ORGANISM(S): Cicer arietinum  

SUBMITTER: Yun Zheng   Ramanjulu Sunkar 

PROVIDER: E-GEOD-62216 | ArrayExpress | 2015-05-08

SECONDARY ACCESSION(S): GSE62216SRP048805PRJNA263491

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

High throughput sequencing of small RNA component of leaves and inflorescence revealed conserved and novel miRNAs as well as phasiRNA loci in chickpea.

Srivastava Sangeeta S   Zheng Yun Y   Kudapa Himabindu H   Jagadeeswaran Guru G   Hivrale Vandana V   Varshney Rajeev K RK   Sunkar Ramanjulu R  

Plant science : an international journal of experimental plant biology 20150309


Among legumes, chickpea (Cicer arietinum L.) is the second most important crop after soybean. MicroRNAs (miRNAs) play important roles by regulating target gene expression important for plant development and tolerance to stress conditions. Additionally, recently discovered phased siRNAs (phasiRNAs), a new class of small RNAs, are abundantly produced in legumes. Nevertheless, little is known about these regulatory molecules in chickpea. The small RNA population was sequenced from leaves and flower  ...[more]

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