Transcriptomics

Dataset Information

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RNA-SEQ analysis of the developing lower urogenital region


ABSTRACT: We will use micro-dissection and FACS techniques to isolate cell types from the developing lower urogenital region and perform a transcriptional analysis using RNA-SEQ. This analysis will determine the transcriptional profile of each cell type, identify compartment specific transcripts, compartment specific transcript isoforms and cell-type specific long-noncoding RNAs. In addition the unbiased nature of RNA-SEQ will potentially identify novel transcripts that have not been annotated in the database. Total RNA was obtained from FACS isolated embryonic day 17.5 bladders using Tg(Upk2-cre)6Xrw x Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo transgenic mice. The long term goal is to generate a transcriptional atlas of developing lower urogenital region.

ORGANISM(S): Mus musculus  

SUBMITTER: Cathy Mendelsohn   Eric W Brunskill  Kerry Schneider  GUDMAP Developers 

PROVIDER: E-GEOD-62310 | ArrayExpress | 2014-12-10

SECONDARY ACCESSION(S): SRP048901GSE62310PRJNA263812

REPOSITORIES: GEO, ArrayExpress, ENA

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