Transcriptomics

Dataset Information

97

Systems analyses reveal shared and diverse attributes of Oct4 regulation in pluripotent cells


ABSTRACT: Epiblast stem cells (EpiSCs) are pluripotent cells that can be isolated and cultured from post implantation embryos. In contrast to embryonic stem cells (ESCs), systematic studies to investigate the genes that maintain pluripotency in EpiSCs have not been reported. Here we combine a genome-wide RNAi screen with genetic interaction, protein localization and protein-level dependency studies to delineate connectivity between factors that control Oct4 expression in EpiSCs and compare the role of these factors to their function in ES cells. We demonstrate the power of this integrative approach by the identification of Tox4 as an interactor of PP1 (Protein Phosphatase 1) and Paf1C, a complex that acts in multiple aspects of RNAPII regulation. Our results indicate that Tox4 cooperates with Paf1C and PP1 to influence the phosphorylation status of the RNAPII CTD tail during transcription and that this function is vital for maintenance of pluripotent cell identity. RNA-seq of Tox4 knockdown in mouse EpiSCs

ORGANISM(S): Mus musculus  

SUBMITTER: Maciej Paszkowski-Rogacz  Li Ding   Frank Buchholz    

PROVIDER: E-GEOD-62357 | ArrayExpress | 2015-08-19

SECONDARY ACCESSION(S): GSE62357SRP048937PRJNA263915

REPOSITORIES: GEO, ArrayExpress, ENA

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