Transcriptomics

Dataset Information

10

Transcription profiling of mouse WAP-SVT/t transgenic animals during breast cancer formation


ABSTRACT: Microarray studies revealed that as a first hit, SV40 T/t-antigen causes deregulation of 462 genes in mammary gland cells (ME-cells) of WAP-SVT/t transgenic animals. The majority of deregulated genes are cell-proliferation specific and Rb-E2F dependent, causing ME-cell proliferation and gland hyperplasia but not breast cancer formation. In the breast tumor cells, a further 207 genes are differentially expressed, most of them belonging to the cell communication category. In tissue culture, breast tumor cells frequently switch off WAP-SVT/t transgene expression and regain the morphology and growth characteristics of normal-ME-cells, although the tumor-revertant cells are aneuploid and only 114 genes regain the expression level of normal-ME-cells. The profile of retransformants shows that only 38 deregulated genes appear to be tumor-relevant and that none of them is considered to be a typical breast cancer gene. Experiment Overall Design: We have analyzed nine mammary gland tissue segments from three normal NMRI, three WAP-SVT/t mice on the first day of lactation and from three WAP-SVT/t breast cancers (583-tumor 1, 585-tumor 2, 597-tumor 3) as well as three distinct breast cancer derived cell lines (ME-A cells, revertant-ME-B cells and ME-B-T/t cells).

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Mus musculus  

DISEASE(S): Breast Cancer

SUBMITTER: Andreas Klein  

PROVIDER: E-GEOD-6246 | ArrayExpress | 2009-04-25

SECONDARY ACCESSION(S): GDS2514GSE6246PRJNA97521

REPOSITORIES: GEO, ArrayExpress

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Publications

Gene expression profiling: cell cycle deregulation and aneuploidy do not cause breast cancer formation in WAP-SVT/t transgenic animals.

Klein Andreas A   Guhl Eva E   Zollinger Raphael R   Tzeng Yin-Jeh YJ   Wessel Ralf R   Hummel Michael M   Graessmann Monika M   Graessmann Adolf A  

Journal of molecular medicine (Berlin, Germany) 20050121 5


Microarray studies revealed that as a first hit the SV40 T/t antigen causes deregulation of 462 genes in mammary gland cells (ME cells) of WAP-SVT/t transgenic animals. The majority of deregulated genes are cell proliferation specific and Rb-E2F dependent, causing ME cell proliferation and gland hyperplasia but not breast cancer formation. In the breast tumor cells a further 207 genes are differentially expressed, most of them belonging to the cell communication category. In tissue culture breas  ...[more]

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