Transcriptomics

Dataset Information

298

Differential gene expression analysis in adult rotation-resistant (RR) and wild-type (WT) western corn rootworm digestive tracts


ABSTRACT: The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) is an important pest of corn (Zea mays) in the US. Annual crop rotation between corn and soybean (Glycine max) disrupts the corn-dependent WCR lifecycle and was widely adopted to manage WCR. However, this strategy selected for a rotation-resistant (RR) variant with reduced ovipositional fidelity to cornfields. Previous studies indicated that RR-WCR adults exhibit greater tolerance of soybean tissue diet, different gut physiology, and host-microbe interactions compared to wild-types (WT). To identify genetic mechanisms underlying these phenotypic changes, a de novo assembly of the WCR adult gut transcriptome was constructed and used for RNA-sequencing analyses on RNA libraries from different WCR phenotypes (RR and WT) fed with corn or soybean diets. Differential gene expression analyses and network-based methods were used to identify gene modules transcriptionally correlated with the RR phenotype. Gene ontology enrichment analyses on these modules were then conducted to understand their potential functions and biological importance. Differential gene expression analyses on RNA libraries from adult guts of different WCR phenotypes (rotation-resistant and wild-type) fed with corn or soybean diets

ORGANISM(S): Diabrotica virgifera virgifera  

SUBMITTER: Joseph Spencer   Matías Curzi  Chia-Ching Chu  Jorge Zavala  Manfredo Seufferheld 

PROVIDER: E-GEOD-62480 | ArrayExpress | 2015-11-23

SECONDARY ACCESSION(S): GSE62480SRP049056

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Patterns of differential gene expression in adult rotation-resistant and wild-type western corn rootworm digestive tracts.

Chu Chia-Ching CC   Zavala Jorge A JA   Spencer Joseph L JL   Curzi Matías J MJ   Fields Christopher J CJ   Drnevich Jenny J   Siegfried Blair D BD   Seufferheld Manfredo J MJ  

Evolutionary applications 20150716 7


The western corn rootworm (WCR,Diabrotica virgifera virgifera LeConte) is an important pest of corn. Annual crop rotation between corn and soybean disrupts the corn-dependent WCR life cycle and is widely adopted to manage this pest. This strategy selected for rotation-resistant (RR) WCR with reduced ovipositional fidelity to corn. Previous studies revealed that RR-WCR adults exhibit greater tolerance of soybean diets, different gut physiology, and host-microbe interactions compared to rotation-s  ...[more]

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