Transcriptomics

Dataset Information

4

Transcription profiling of rat adrenal glands from normoxic and hypoxic neonatal animals


ABSTRACT: We hypothesize that changes in adrenal gene expression mediate the increased plasma corticosterone and steroidogenesis in rat pups exposed to hypoxia from birth. Experiment Overall Design: In the current study, rat pups (with their dams) were exposed to hypoxia from birth and compared to pups from normoxic dams fed ad libitum. Adrenal glands were collected from normoxic and hypoxic pups at PD7and pooled (N=24/sample; 4 samples per treatment group) for total RNA extraction. Microarray analysis was performed, followed by verification with real-time PCR. Furthermore, the expression of selected genes involved in adrenal function was analyzed by real-time PCR, regardless of microarray results.

INSTRUMENT(S): 418 [Affymetrix]

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Eric David Bruder  

PROVIDER: E-GEOD-6249 | ArrayExpress | 2008-06-14

SECONDARY ACCESSION(S): GSE6249PRJNA97523

REPOSITORIES: GEO, ArrayExpress

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Publications

Microarray and real-time PCR analysis of adrenal gland gene expression in the 7-day-old rat: effects of hypoxia from birth.

Bruder Eric D ED   Lee Julie J JJ   Widmaier Eric P EP   Raff Hershel H  

Physiological genomics 20070109 2


We hypothesize that changes in adrenal gene expression mediate the increased plasma corticosterone and steroidogenesis in rat pups exposed to hypoxia from birth. In the current study, rat pups (with their dams) were exposed to hypoxia from birth and compared with pups from normoxic dams fed ad libitum or pair fed to match the decreased maternal food intake that occurs during hypoxia. Microarray analysis was performed, followed by verification with real-time PCR. Furthermore, the expression of se  ...[more]

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