Transcriptomics

Dataset Information

102

Gene expression signature of Gata6 positive epidermal cells.


ABSTRACT: To characterize gene expression in Gata6 positive epidermal cells we analyzed a Gata6 reporter mouse in which the endogenous Gata6 promoter drives expression of mTomato. We performed flow cytometry followed by transcriptome analysis. We compared four subpopulations of telogen epidermal cells: Gata6+/Itga6+ cells, Gata6+/itga6- cells, CD34+/Itga6+ cells (which are Gata6-) and all remaining Itga6+ cells (Gata6-/CD34-). The RNA was isolated from age and sex matched mice. We compared four subpopulations of telogen epidermal cells: Gata6+/Itga6+ cells, Gata6+/itga6- cells, CD34+/Itga6+ cells (which are Gata6-) and all remaining Itga6+ cells (Gata6-/CD34-). The RNA was isolated from age and sex matched mice. Three biological replicates for each cell population were analyzed

ORGANISM(S): Mus musculus  

SUBMITTER: Fiona Watt   Giacomo Donati 

PROVIDER: E-GEOD-62606 | ArrayExpress | 2017-05-16

SECONDARY ACCESSION(S): GSE62606PRJNA264529

REPOSITORIES: GEO, ArrayExpress

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Publications

Wounding induces dedifferentiation of epidermal Gata6+ cells and acquisition of stem cell properties.

Donati Giacomo G   Rognoni Emanuel E   Hiratsuka Toru T   Liakath-Ali Kifayathullah K   Hoste Esther E   Kar Gozde G   Kayikci Melis M   Russell Roslin R   Kretzschmar Kai K   Mulder Klaas W KW   Teichmann Sarah A SA   Watt Fiona M FM  

Nature cell biology 20170515 6


The epidermis is maintained by multiple stem cell populations whose progeny differentiate along diverse, and spatially distinct, lineages. Here we show that the transcription factor Gata6 controls the identity of the previously uncharacterized sebaceous duct (SD) lineage and identify the Gata6 downstream transcription factor network that specifies a lineage switch between sebocytes and SD cells. During wound healing differentiated Gata6+ cells migrate from the SD into the interfollicular epiderm  ...[more]

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