Transcriptomics

Dataset Information

300

The Partitioning of RNAs in sperm


ABSTRACT: A diverse pool of RNAs remain encapsulated within the transcriptionally and translationally silent spermatozoon. These transcripts persist within the male gamete despite the dramatic reduction in cellular volume achieved through expulsion of the cytoplasm and quite possibly the nucleoplasm. The precise location of RNAs retained within the sperm cell remains largely unknown. However, early evidence suggested that many are embedded within the nucleus (1). To discern the global pattern of transcript compartmentalization in sperm, total RNA was extracted from whole mouse spermatozoa and detergent demembranated nuclei fractionated through a sucrose gradient. Isolated RNAs were subjected to RNA-sequencing (RNA-seq) and their abundance used to infer localization. Transcripts enriched in the unfractionated cells were related to the production and function of mitochondria and surprisingly, exosomes. The absence of these extracellular vesicles associated RNAs within the inner-nuclear compartment was suggestive of an origin other than sperm. This contributes to the growing evidence for sperm-bound exosomes rich in RNA. In comparison, the majority of the remaining sperm RNAs were associated with the nucleus. This included the abundant fragmented ribosomal transcripts which likely persist between the nuclear envelope and the perinuclear theca. The spermatozoal inner-nuclear compartment was also enriched in repetitive transcribed sequences. This included LINE elements and simple repeat sequences both of which have been shown to contribute to chromatin structure in other cell types suggesting that they may serve parallel roles in the spermatozoon. RNA-seq analysis of whole mouse sperm and fractionated nuclei

ORGANISM(S): Mus musculus  

SUBMITTER: Graham Johnson   Stephen A Krawet  Graham D Johnson 

PROVIDER: E-GEOD-62874 | ArrayExpress | 2015-07-08

SECONDARY ACCESSION(S): GSE62874SRP049420PRJNA265872

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Chromatin and extracellular vesicle associated sperm RNAs.

Johnson Graham D GD   Mackie Paula P   Jodar Meritxell M   Moskovtsev Sergey S   Krawetz Stephen A SA  

Nucleic acids research 20150613 14


A diverse pool of RNAs remain encapsulated within the transcriptionally silent spermatozoon despite the dramatic reduction in cellular and nuclear volume following cytoplasm/nucleoplasm expulsion. The impact of this pronounced restructuring on the distribution of transcripts inside the sperm essentially remains unknown. To define their compartmentalization, total RNA >100 nt was extracted from sonicated (SS) mouse spermatozoa and detergent demembranated sucrose gradient fractionated (Cs/Tx) sper  ...[more]

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