Genomics

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Base-resolution detection of N4-methylcytosine in genomic DNA using 4mC-TAB-seq


ABSTRACT: N4-methylcytosine is a major DNA modification integral to restriction-modification (R-M) systems in bacterial genomes. Here we describe 4mC-Tet-Assisted Bisulfite-sequencing (4mC-TAB-seq), a method that accurately and rapidly reveals the genome-wide locations of N4-methylcytosines at single-base resolution. By coupling Tet-mediated oxidation with a modified sodium bisulfite conversion reaction, unmodified cytosines and 5-methylcytosines are read out as thymines, whereas N4-methylcytosines are read out as cytosines revealing their positions throughout the genome. 4mC-TAB-seq

SUBMITTER: Robert J Schmitz  

PROVIDER: E-GEOD-63371 | ArrayExpress | 2015-07-11

SECONDARY ACCESSION(S): GSE63371SRP049960PRJNA267586

REPOSITORIES: GEO, ArrayExpress, ENA

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