Transcriptomics

Dataset Information

297

Pre-mRNA Splicing is Facilitated by an Optimal RNA Polymerase II Elongation Rate


ABSTRACT: Transcription and pre-mRNA alternative splicing was analyzed by in isogenic human HEK293 cell lines that inducibly express a-amanitin resistant mutants of the RNA polymerase II large subunit with slow and fast elongation rates. Alternative splicing was analyzed by, RNA-seq and RASL-seq of polyA+ RNA from a-amanitin treated cells; transcription elongation rate was analyzed by BrUTP-labelled GRO-seq of a-amanitin treated cells at time points after release from a DRB (5,6-dichloro-1-bold beta-D-ribofuranosylbenzimidazole) block.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hyunmin Kim   David Bentley  David L. Bentley  Xiang-Dong Fu 

PROVIDER: E-GEOD-63375 | ArrayExpress | 2014-11-25

SECONDARY ACCESSION(S): SRP050215GSE63375PRJNA268365

REPOSITORIES: GEO, ArrayExpress, ENA

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