Transcriptomics

Dataset Information

300

RNA expression analysis upon JMJD1C depletion


ABSTRACT: The AML1-ETO fusion protein, a transcription factor generated by the t(8;21) translocation in acute myeloid leukaemia (AML), dictates a leukemic program by increasing self-renewal and inhibiting differentiation. Here we demonstrate that the histone demethylase JMJD1C functions as a co-activator for AML1-ETO and is required for its transcriptional program. JMJD1C is directly recruited by AML1-ETO to its target genes and regulates their expression by maintaining low H3K9me2 levels. Analyses in JMJD1C knockout mice also establish a JMJD1C requirement for AML1-ETO’s ability to increase proliferation. We also show a critical role for JMJD1C in the survival of multiple human AML cell lines, suggesting that it is required for leukemic programs in different AML cell types through its association with key transcription factors. Examination of RNA expression when Kasumi-1 cells are treated with control shRNA or two different JMJD1C shRNAs; in duplicate. Please note that the 'shAML1_ETO_vs_shControl.all_gene_exp.tb.txtl' was generated comparing control and shRNA treated RNA abundance-using previously published data [GSE43834; GSM1071857 and GSM1071852].

ORGANISM(S): Homo sapiens  

SUBMITTER: Mo Chen   Robert G Roeder 

PROVIDER: E-GEOD-63485 | ArrayExpress | 2015-11-02

SECONDARY ACCESSION(S): SRP050058GSE63485PRJNA268020

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

JMJD1C is required for the survival of acute myeloid leukemia by functioning as a coactivator for key transcription factors.

Chen Mo M   Zhu Nan N   Liu Xiaochuan X   Laurent Benoit B   Tang Zhanyun Z   Eng Rowena R   Shi Yang Y   Armstrong Scott A SA   Roeder Robert G RG  

Genes & Development 20151001 20


RUNX1-RUNX1T1 (formerly AML1-ETO), a transcription factor generated by the t(8;21) translocation in acute myeloid leukemia (AML), dictates a leukemic program by increasing self-renewal and inhibiting differentiation. Here we demonstrate that the histone demethylase JMJD1C functions as a coactivator for RUNX1-RUNX1T1 and is required for its transcriptional program. JMJD1C is directly recruited by RUNX1-RUNX1T1 to its target genes and regulates their expression by maintaining low H3K9 dimethyl (H3  ...[more]

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