Genomics

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An alternative pluripotent state confers interspecies chimaeric competency [WGBS]


ABSTRACT: In this study, we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as region selective epiblast stem cells (rsEpiSCs), could be efficiently derived from different stages of the early embryo. rsEpiSCs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed whole-genome bisulfite sequencing (WGBS) experiments to compare the DNA methylation landscapes of conventional EpiSCs and rsEpiSCs. Compare the DNA methylation profiles in 2 pluripotent stem cell types (LP-EpiSCs and conventional EpiSCs) in mouse. Two replicates are examined for each cell type.

ORGANISM(S): Mus musculus  

SUBMITTER: Zhuzhu Zhang   Chongyuan Luo  Christopher Benner  Yupeng He 

PROVIDER: E-GEOD-63568 | ArrayExpress | 2015-05-06

SECONDARY ACCESSION(S): SRP050145GSE63568PRJNA268217

REPOSITORIES: GEO, ArrayExpress, ENA

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Pluripotency, the ability to generate any cell type of the body, is an evanescent attribute of embryonic cells. Transitory pluripotent cells can be captured at different time points during embryogenesis and maintained as embryonic stem cells or epiblast stem cells in culture. Since ontogenesis is a dynamic process in both space and time, it seems counterintuitive that these two temporal states represent the full spectrum of organismal pluripotency. Here we show that by modulating culture paramet  ...[more]

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