Transcriptomics

Dataset Information

0

GM-CSF induced gene-regulation in human monocytes


ABSTRACT: Human and murine studies showed that granulocyte macrophage colony-stimulating factor (GM-CSF) exerts beneficial effects in intestinal inflammation. To explore whether GM-CSF mediates its effects via monocytes, we analyzed effects of GM-CSF on monocytes in vitro and assessed the immunomodulatory potential of GM-CSF-activated monocytes (GMaM). We used microarray technology and functional assays to characterize GMaM in vitro and used a mouse model of colitis to study GMaM functions in vivo. Peripheral blood monocytes where cultured 16 h with media containing AB serum (control monocytes) or media containing 10 ng/mL GM-CSF and AB serum (GM-CSF activated monocytes). In three independent experiments, total RNA from GMaM and control monocytes was isolated and processed for microarray hybridization.

ORGANISM(S): Homo sapiens  

SUBMITTER: Dominik Bettenworth  David Schwarzmaier   Anne Brockhausen   Karoline Walscheid   Dirk Foell   Toni Weinhage   Johannes Roth   Markus Brückner   Georg Varga   Jan Däbritz   Jan M Ehrchen   Matthias Ross   Timo Wirth    

PROVIDER: E-GEOD-63662 | ArrayExpress | 2014-11-27

SECONDARY ACCESSION(S): GSE63662PRJNA268650

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-06-01 | E-GEOD-47621 | ArrayExpress
2014-05-01 | E-MTAB-2212 | ArrayExpress
2010-06-16 | E-GEOD-19236 | ArrayExpress
2012-05-20 | E-GEOD-37029 | ArrayExpress
2012-03-20 | E-MTAB-791 | ArrayExpress
2012-05-20 | E-GEOD-37030 | ArrayExpress
2012-04-12 | E-GEOD-27792 | ArrayExpress
2010-06-20 | E-GEOD-18654 | ArrayExpress
2015-04-21 | E-GEOD-68061 | ArrayExpress
2008-10-25 | E-GEOD-12773 | ArrayExpress