Transcriptomics

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The RNA binding protein Arrest (Aret) regulates myofibril maturation in Drosophila flight muscle


ABSTRACT: In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between cytoplasm and nuclei, and is essential for myofibril maturation and sarcomere growth of IFMs. Molecularly, Aret regulates IFM-specific transcription and splicing of various sarcomeric targets, including Stretchin and wupA (TnI), and thus maintains muscle fiber integrity. As Aret and its sarcomeric targets are evolutionarily conserved, similar principles may regulate mammalian muscle morphogenesis. 9 samples from Drosophila melanogaster were analyzed in duplicate: control dissected wildtype flight muscle at 30h APF, 72h APF and 0 day adult, jump muscle and whole leg from 1d adult and RNAi/mutant conditions for salm (1d flight muscle) and aret (30h, 72h and 1d flight muscle)

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Alexander Stark   Assa Yeroslaviz  Daniel Gerlach  Bianca Habermann  Maria L Spletter  Maria Lynn Spletter  Frank Schnorrer 

PROVIDER: E-GEOD-63707 | ArrayExpress | 2014-12-23

SECONDARY ACCESSION(S): SRP050336GSE63707PRJNA268841

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The RNA-binding protein Arrest (Bruno) regulates alternative splicing to enable myofibril maturation in Drosophila flight muscle.

Spletter Maria L ML   Barz Christiane C   Yeroslaviz Assa A   Schönbauer Cornelia C   Ferreira Irene R S IR   Sarov Mihail M   Gerlach Daniel D   Stark Alexander A   Habermann Bianca H BH   Schnorrer Frank F  

EMBO reports 20141222 2


In Drosophila, fibrillar flight muscles (IFMs) enable flight, while tubular muscles mediate other body movements. Here, we use RNA-sequencing and isoform-specific reporters to show that spalt major (salm) determines fibrillar muscle physiology by regulating transcription and alternative splicing of a large set of sarcomeric proteins. We identify the RNA-binding protein Arrest (Aret, Bruno) as downstream of salm. Aret shuttles between the cytoplasm and nuclei and is essential for myofibril matura  ...[more]

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