Transcriptomics

Dataset Information

2

Microarray analyses of ESAM positive cells in the CD34(+) CD38(-) fraction of collagenase-treated bones


ABSTRACT: We previously identified endothelial cell-selective adhesion molecule (ESAM) as a novel functional marker of hematopoietic stem cells (HSCs) in mice. To examine ESAM expression in human, we analyzed diverse HSC sources using flow cytometry. From mononuclear cells of collagenase-treated human hip bones, we obtained two ESAM positive populations in CD34(+) CD38(-) fraction, referred to as ESAM(High) and ESAM(Bright). Then we conducted microarray analyses comparing gene expression signatures between these two populations. Trabecular tissues of the femora were treated with collagenase IV and DNase. Mononuclear cells were collected, and CD34(+) CD38(-) ESAM(High) or ESAM(Bright) cells were sorted.

ORGANISM(S): Homo sapiens  

SUBMITTER: Michiko Ichii   Takafumi Yokota  Tomohiko Ishibashi 

PROVIDER: E-GEOD-63877 | ArrayExpress | 2014-12-05

SECONDARY ACCESSION(S): GSE63877PRJNA269303

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

| PRJNA269303 | ENA
2017-10-03 | E-MTAB-5456 | ArrayExpress
2005-09-08 | E-EMBL-1 | ArrayExpress
2010-06-28 | GSE22601 | GEO
2015-03-01 | E-GEOD-45249 | ArrayExpress
| GSE45249 | GEO
2015-09-22 | E-GEOD-35008 | ExpressionAtlas
2015-04-23 | E-GEOD-68172 | ArrayExpress
2016-02-25 | E-GEOD-76272 | ArrayExpress
2012-09-05 | E-GEOD-35010 | ArrayExpress