Dataset Information


Increased CD226 Expression on CD8+ T cells in Patients with Systemic Sclerosis

ABSTRACT: Systemic sclerosis (SSc) is an autoimmune disease characterized by vascular damage, and fibrosis of the skin and internal organs. Because activated and oligoclonally expanded CD8+ T cells can be detected in peripheral blood and lung of SSc patients, effector memory CD8+ T cells may play a critical role for organ involvement in SSc; however, the pathogenic functions of effector memory CD8+ T cells remain incompletely understood. Here we performed DNA microarray analysis of the sort-purified effector memory CD8+ T cells from SSc patients and healthy controls, and showed that the expression of genes related to immune response and cell adhesion, including CD226 (also known as DNAX accessory molecule-1, DNAM-1), was significantly altered. Moreover, detailed analysis of CD226 revealed that CD226highCD8+ T cells were increased in SSc patients (mean, 50.7%) compared with healthy controls (32.9%) and were appreciably associated with the severity of skin sclerosis and interstitial lung disease. Further, CD226highCD8+ T cells from SSc patients produced abundant IL-13 and were positively correlated with the cytotoxic capacity of CD8+ T cells against HUVECs. Finally, the neutralization of CD226 impaired IL-13 production and cytolysis against HUVECs. These findings indicate that upregulated CD226 expression on CD8+ T cells reflects disease severity and are involved in SSc pathogenesis via the production of profibrotic IL-13 and endothelial cell injury, and that CD226 may be a useful target in the treatment of SSc. We first purified effector memory CD8+ T cells (CD3+CD8+CD45RO+CD62L+ cells) from peripheral blood by cell sorting and subsequently performed cDNA microarray analysis of the sort-purified effector memory CD8+ T cells from 9 SSc patients and 5 healthy controls.

ORGANISM(S): Homo sapiens  

SUBMITTER: Masahiro Ayano   Hiroshi Tsukamoto 

PROVIDER: E-GEOD-63903 | ArrayExpress | 2014-12-06



Similar Datasets

2016-09-07 | E-GEOD-76796 | ArrayExpress
2016-09-07 | E-GEOD-76785 | ArrayExpress
2012-12-31 | E-GEOD-34562 | ArrayExpress
2014-06-03 | E-GEOD-50764 | ArrayExpress
2008-06-16 | E-GEOD-8678 | ArrayExpress
2010-02-17 | E-GEOD-19825 | ArrayExpress
2014-12-08 | E-GEOD-63850 | ArrayExpress
| GSE63850 | GEO
2010-11-23 | E-GEOD-25502 | ArrayExpress
| GSE19825 | GEO