Genomics

Dataset Information

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Brg1 Modulates Enhancer Activation and Polycomb-mediated Repression in Mesoderm Differentiation [ChIP-exo]


ABSTRACT: We investigated the genome-wide occupancy changes in normal and Brg1-deleted mesoderm differentiation of mouse embryonic stem cells of chromatin regulators and histone modifications. ChIP exo for BRG1 in 2 conditions: Day4 THF (Control) and Day4 4OHT (Brg1-deleted). 1-2 replicates per condition.

ORGANISM(S): Mus musculus  

SUBMITTER: Jeffrey Alexander  Swetansu Hota   Jeffrey Michael Alexander    

PROVIDER: E-GEOD-63976 | ArrayExpress | 2015-03-01

SECONDARY ACCESSION(S): SRP050970GSE63976PRJNA269676

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Brg1 modulates enhancer activation in mesoderm lineage commitment.

Alexander Jeffrey M JM   Hota Swetansu K SK   He Daniel D   Thomas Sean S   Ho Lena L   Pennacchio Len A LA   Bruneau Benoit G BG  

Development (Cambridge, England) 20150326 8


The interplay between different levels of gene regulation in modulating developmental transcriptional programs, such as histone modifications and chromatin remodeling, is not well understood. Here, we show that the chromatin remodeling factor Brg1 is required for enhancer activation in mesoderm induction. In an embryonic stem cell-based directed differentiation assay, the absence of Brg1 results in a failure of cardiomyocyte differentiation and broad deregulation of lineage-specific gene express  ...[more]