Transcriptomics

Dataset Information

30

Expression data (U133 Plus 2.0) from fibroblast like synoviocytes from patients with rheumatoid arthritis (RA-FLS) stimulated by TL1A


ABSTRACT: TNF-like ligand 1A (TL1A) is a member of TNF receptor superfamily and involved in the pathogenesis of autoimmune diseases by inducing apoptosis via intracellular death domain or promoting inflammation through the activation of NFκB by binding to its specific receptor death receptor 3 (DR3). Meanwhile, decoy receptor 3 (DcR3) competitively binds soluble TL1A in addition to Fas-ligand (FasL) and LIGHT and inhibits the signaling of TL1A via DR3. DcR3 overexpressed in rheumatoid synovial fibroblasts (RA-FLS) stimulated with inflammatory cytokines such as TNFα or IL-1β inhibits Fas-induced apoptosis. In contrast, DcR3 inhibited cell proliferation induced by inflammatory cytokines via membrane-bound TL1A expressed on RA-FLS. Therefore, TL1A-DcR3/DR3 signaling may be involved in the pathogenesis of RA by modulating apoptosis and proliferation of RA-FLS. We hypothesized that TL1A regulates the gene expression in RA-FLS. We used to search for genes in which expression in RA-FLS is regulated by the ligation of TL1A. RA-FLS were obtained from 4 RA patients (sample1-4). Each sample was incubated with either 1.0 μg/ml recombinant human TL1A protein or phosphate buffered saline (PBS) diluted with serum-free Opti-MEM medium as non-stimulated control for 12 hours at 37°C with 5% CO2. Gene expression in RA-FLS stimulated by TL1A was compared with that of their respective non-stimulated controls.

ORGANISM(S): Homo sapiens  

SUBMITTER: Koji Fukuda  Toshihisa Maeda   Shinya Hayashi   Yasushi Miura   Masahiro Kurosaka    

PROVIDER: E-GEOD-63995 | ArrayExpress| 2015-01-02

SECONDARY ACCESSION(S): GSE63995PRJNA269733

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-06-03 | E-GEOD-45665 | ArrayExpress
2014-06-03 | E-GEOD-49604 | ArrayExpress
2015-01-17 | E-GEOD-64922 | ArrayExpress
| GSE49604 | GEO
| GSE91026 | GEO
2018-03-19 | E-MTAB-6457 | ArrayExpress
| GSE65908 | GEO
2014-06-02 | E-GEOD-15615 | ArrayExpress
2011-10-18 | E-MEXP-1970 | ArrayExpress
| GSE95126 | GEO