Transcriptomics,Genomics

Dataset Information

299

Nucleoporin Nup153 Regulates Stem Cell Pluripotency through Gene Silencing


ABSTRACT: Nucleoporins (Nups) are a family of proteins best known as the constituent building blocks of nuclear pore complexes (NPCs), the transport channels that mediate nuclear transport. Recent evidence suggest that several Nups have additional roles in controlling the activation and silencing of developmental genes, however, the mechanistic details of these functions remain poorly understood. Here, we show that depletion of Nup153 in mouse embryonic stem cells (mESCs) causes the de-repression of developmental genes and induction of early differentiation. This loss of pluripotency is not associated with defects in global nucleo-cytoplasmic transport activity. Instead, Nup153 binds to the transcriptional start site (TSS) of developmental genes and mediates the recruitment of the polycomb repressive complex 1 (PRC1) to its target loci. Our results reveal a nuclear transport-independent role of Nup153 in maintaining stem cell pluripotency and introduce a role of NPC proteins in mammalian epigenetic gene silencing. RNA-seq, ChIP-Seq, and DamID-Seq for Nup153, Oct4, and key chromatin regulators in mouse ES cells and neural progenitors

ORGANISM(S): Mus musculus  

SUBMITTER: Martin W Hetzer   Christopher Benner  Filipe V Jacinto 

PROVIDER: E-GEOD-64008 | ArrayExpress | 2015-06-19

SECONDARY ACCESSION(S): SRP050985GSE64008PRJNA269747

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing.

Jacinto Filipe V FV   Benner Chris C   Hetzer Martin W MW  

Genes & development 20150616 12


Nucleoporins (Nups) are a family of proteins best known as the constituent building blocks of nuclear pore complexes (NPCs), membrane-embedded channels that mediate nuclear transport across the nuclear envelope. Recent evidence suggests that several Nups have additional roles in controlling the activation and silencing of developmental genes; however, the mechanistic details of these functions remain poorly understood. Here, we show that depletion of Nup153 in mouse embryonic stem cells (mESCs)  ...[more]

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