Transcriptomics

Dataset Information

31

RNA-seq of autistic and control cortex


ABSTRACT: Purpose: The goal of this study was to assess the status of splicing changes in microexons in the cortex of individuals with autism. Methods: We performed RiboZero Gold (rRNA depleted) 50bp PE RNA-seq in a larger set of case and control samples to define 12 autism and 12 control samples showing the greatest global differential gene expression change. These samples, which show differential expression of the splicing regulator SRRM4, were used to evaluate global splicing changes. Results: Within these samples, 126 of 504 (30%) detected alternative microexons display a mean ΔPSI > 10 between ASD and control subjects of which 113 (90%) also display neural-differential regulation. By contrast, only 825 of 15,405 (5.4%) longer (i.e. >27 nt) exons show such misregulation, of which 285 (35%) correspond to neural-regulated exons. Notably, we also observe significantly higher correlations between microexon inclusion and nSR100 mRNA expression levels across the stratified ASD samples and controls, for those microexons regulated by nSR100 relative to those microexons that are not regulated by this factor (p=1.4×10-7, Wilcoxon Sum Rank test). Conclusions: These data suggest microexon regulation is a potentially important mechanism underlying ASD and likely other neurodevelopmental disorders 12 case samples representing a more extreme autism gene expression signature and 12 representative controls; raw data have been submitted to dbGaP

ORGANISM(S): Homo sapiens  

SUBMITTER: Neelroop Parikshak  Neelroop N Parikshak   Daniel H Geschwind    

PROVIDER: E-GEOD-64018 | ArrayExpress| 2015-01-02

SECONDARY ACCESSION(S): GSE64018

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

| phs000872 | dbGaP
2016-04-07 | E-GEOD-30573 | ExpressionAtlas
2016-04-05 | E-GEOD-30573 | ArrayExpress
2011-05-18 | E-GEOD-28521 | ArrayExpress
2011-05-01 | GSE28521 | GEO
2011-06-08 | GSE29576 | GEO
2016-02-27 | E-GEOD-76852 | ArrayExpress
2015-09-11 | E-GEOD-72149 | ArrayExpress
2018-11-16 | E-MTAB-7351 | ArrayExpress
2014-05-02 | E-GEOD-21086 | ArrayExpress