Transcriptomics,Multiomics

Dataset Information

2

Transcription profiling by high throughput sequencing of tomato leaves treated with and without the DC3000 pathogen and nightly red light treatment


ABSTRACT: Background: Plants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilitate the elucidation of this mechanism. Results: In this study, we observed diurnal changes in tomato resistance to Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), with the greatest susceptibility before midnight. Nightly light treatment, particularly red light treatment, significantly enhanced the resistance; this effect was correlated with increased salicylic acid (SA) accumulation and defence-related gene transcription. RNA-seq analysis revealed that red light induced a set of circadian rhythm-related genes involved in the phytochrome and SA-regulated resistance response. The biosynthesis and signalling pathways of multiple plant hormones (auxin, SA, jasmonate, and ethylene) were co-ordinately regulated following Pto DC3000 infection and red light, and the SA pathway was most significantly affected by red light and Pto DC3000 infection. This result indicates that SA-mediated signalling pathways are involved in red light-induced resistance to pathogens. Importantly, silencing of nonexpressor of pathogensis-related genes 1 (NPR1) partially compromised red light-induced resistance against Pto DC3000. Furthermore, sets of genes involved in redox homeostasis (respiratory burst oxidase homologue, RBOH; glutathione S-transferases, GSTs; glycosyltransferase, GTs), calcium (calmodulin, CAM; calmodulin-binding protein, CBP), and defence (polyphenol oxidase, PPO; nudix hydrolase1, NUDX1) as well as transcription factors (WRKY18, WRKY53, WRKY60, WRKY70) and cellulose synthase were differentially induced at the transcriptional level by red light in response to pathogen challenge. Conclusions: Taken together, our results suggest that there is a diurnal change in susceptibility to Pto DC3000 with greatest susceptibility in the evening. The red light induced-resistance to Pto DC3000 at night is associated with enhancement of the SA pathway, cellulose synthase, and reduced redox homeostasis. Four treatments including control, three biological replicates each treatment

REANALYSED by: E-GEOD-64087

OTHER RELATED OMICS DATASETS IN: PRJNA270083

ORGANISM(S): Solanum Lycopersicum

SUBMITTER: Guofu Zhou   Yan-Ling Yin  Meng-Meng Wang  Kai Shi  Yan-Hong Zhou  Sheng Peng  You-Xin Yang  Jing-Quan Yu  Youxin Yang  Xiao-Jian Xia  Eugen Onac 

PROVIDER: E-GEOD-64087 | ArrayExpress | 2015-09-14

REPOSITORIES: ArrayExpress

Dataset's files

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E-GEOD-64087.additional.1.zip Other
E-GEOD-64087.idf.txt Idf
E-GEOD-64087.sdrf.txt Txt
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Publications

RNA-seq analysis reveals the role of red light in resistance against Pseudomonas syringae pv. tomato DC3000 in tomato plants.

Yang You-Xin YX   Wang Meng-Meng MM   Yin Yan-Ling YL   Onac Eugen E   Zhou Guo-Fu GF   Peng Sheng S   Xia Xiao-Jian XJ   Shi Kai K   Yu Jing-Quan JQ   Zhou Yan-Hong YH  

BMC genomics 20150225


<h4>Background</h4>Plants attenuate their responses to a variety of bacterial and fungal pathogens, leading to higher incidences of pathogen infection at night. However, little is known about the molecular mechanism responsible for the light-induced defence response; transcriptome data would likely facilitate the elucidation of this mechanism.<h4>Results</h4>In this study, we observed diurnal changes in tomato resistance to Pseudomonas syringae pv. tomato DC3000 (Pto DC3000), with the greatest s  ...[more]

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