Dataset Information


Intra-sample heterogeneity of sperm DNA methylation

ABSTRACT: Genome wide DNA methylation profiling of 2 distinct subpopulations of sperm within a single ejaculate. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs. Samples were provided by 20 normozoospermic individuals. A density gradient centrifugation was performed on each samples to yeild two distict populations for each ejaculate (one from the 90% isolate layer high quality sperm and one from the 35% isolate layer low quality sperm of the column). Bisulphite converted DNA from the 40 samples were hybridised to the Illumina Infinium 450k Human Methylation Beadchip

ORGANISM(S): Homo sapiens  

SUBMITTER: Timothy G Jenkins  Douglas T Carrell   Douglas Carrell    

PROVIDER: E-GEOD-64096 | ArrayExpress | 2015-02-08



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