Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Steffen Sass Jelena R Linnemann Christina H Scheel Martin Irmler Lisa K Meixner Uwe J Kloos Benjamin Hirschi Christian Gabka Harald S Bartsch Johannes Beckers Haruko Miura Karl Sotlar Fabian Theis
PROVIDER: E-GEOD-64248 | ArrayExpress | 2015-08-19
SECONDARY ACCESSION(S): GSE64248PRJNA270533
REPOSITORIES: GEO, ArrayExpress
Development (Cambridge, England) 20150612 18
We present an organoid regeneration assay in which freshly isolated human mammary epithelial cells are cultured in adherent or floating collagen gels, corresponding to a rigid or compliant matrix environment. In both conditions, luminal progenitors form spheres, whereas basal cells generate branched ductal structures. In compliant but not rigid collagen gels, branching ducts form alveoli at their tips, express basal and luminal markers at correct positions, and display contractility, which is re ...[more]