Transcriptomics

Dataset Information

49

Expression data from kidneys of rats with and without glomerulonephritis


ABSTRACT: We investigated a glomerulonephritis (GN) model in rats induced by nephrotoxic serum (NTS) which contains antibodies against the glomerular basement membrane (GBM). The anti-GBM GN model in rats is widely used since its biochemical and histopathological characteristics are similar to crescentic nephritis and Goodpasture's disease in humans. Male Wistar Kyoto (WKY) and Sprague Dawley (SD) rats were dosed once with 1, 2.5 and 5 ml/kg nephrotoxic serum (NTS) or 1.5 and 5 ml/kg NTS, respectively. GN and tubular damage were observed histopathologically in all treated rats after 14 days. To obtain insight into molecular processes during GN pathogenesis, mRNA expression was investigated in WKY and SD kidneys. The immunopathological processes during GN are still not fully understood and likely involve both innate and adaptive immunity. In the present study, several hundred mRNAs were found deregulated, which functionally were mostly associated with inflammation and regeneration. The β-chain of the major histocompatibility complex class II RT1.B (Rt1-Bb) and complement component 6 (C6) were identified as two mRNAs differentially expressed between WKY and SD rat strains which could be related to known different susceptibilities to NTS of different rat strains; both were increased in WKY and decreased in SD rats. Increased Rt1-Bb expression in WKY rats could indicate a stronger and more persistent cellular reaction of the adaptive immune system in this strain, in line with findings indicating adaptive immune reactions during GN. The complement cascade is also known to be essential for GN development, especially terminal cascade products like C6. Two different rat strains (WKY and SD) were dosed with different doses of NTS and after 14 days rats were euthanized and kidneys were removed for RNA extraction and hybridization on Affymetrix microarrays. We sought to analyze the deregulation of gene expression during NTS-induced glomerulonephritis and to compare the effect in the two rat strains.

ORGANISM(S): Rattus norvegicus  

SUBMITTER: Heidrun Christine Ellinger-Ziegelbauer  Mira Pavkovic   Anna-Lena Frisk   Bjoern Riefke   Ina Groetike   Heidrun Ellinger-Ziegelbauer    

PROVIDER: E-GEOD-64265 | ArrayExpress | 2015-08-19

SECONDARY ACCESSION(S): GSE64265PRJNA270575

REPOSITORIES: GEO, ArrayExpress

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Publications

Glomerulonephritis-Induced Changes in Urinary and Kidney MicroRNA Profiles in Rats.

Pavkovic Mira M   Riefke Björn B   Frisk Anna-Lena AL   Gröticke Ina I   Ellinger-Ziegelbauer Heidrun H  

Toxicological sciences : an official journal of the Society of Toxicology 20150309 2


MicroRNAs (miRNAs) regulate gene expression post-transcriptionally and thus are involved in various physiological and pathological states. Due to their stability in biofluids miRNAs have also been proposed as biomarkers (BMs) for tissue injury. We investigated the usefulness of urinary miRNAs for detection of site-specific renal damage in an antiglomerular basement membrane glomerulonephritis (GN) model in rats by comparing GN-induced urinary miRNAs profiles to traditional and newer protein BMs,  ...[more]

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