Transcriptomics

Dataset Information

41

Time-dependent global gene expression profile of RAW264.7 cells infected with Mycobacterium tuberculosis H37Rv (Mtb)


ABSTRACT: Transcriptional profiling of RAW264.7 cells infected with M. tuberculosis H37Rv at an MOI of 10 performed 4 and 24 hours post-infection. RAW264.7 cells were infected with Mtb for 4 hours at an MOI of 10. Cells were washed and treated with gentamycin for 2 hours in order to remove adhered bacteria. Incubation was continued further for 4 and 24 hours followed by total RNA isolation. Total RNA was labelled with Agilent’s quick-Amp labelling kit (p/n: 5190-0444) to generate fluorescent complementary RNA by using T7 promoter based linear amplification. The control sample was labelled with Cy3 while the infected samples were labelled with Cy5 and hybridized to an Agilent oligo microarray kit.

ORGANISM(S): Mus musculus  

SUBMITTER: Joyoti Basu  Zhumur Ghosh   Manikuntala Kundu    

PROVIDER: E-GEOD-64427 | ArrayExpress | 2015-01-02

SECONDARY ACCESSION(S): GSE64427PRJNA271000

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

| GSE72591 | GEO
2015-09-05 | E-GEOD-72591 | ArrayExpress
2016-07-10 | E-GEOD-81675 | ArrayExpress
2011-06-10 | E-GEOD-29584 | ArrayExpress
2016-04-09 | E-GEOD-72077 | ArrayExpress
2014-05-02 | E-MEXP-2310 | ArrayExpress
| GSE94821 | GEO
| GSE76355 | GEO
2015-04-27 | E-GEOD-61562 | ArrayExpress
| GSE94843 | GEO